Dataset related to the article-Fenretinide treatment accelerates atherosclerosis development in apoE‐deficient mice in spite of beneficial metabolic effects
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This record contains raw data related to the article "Fenretinide treatment accelerates atherosclerosis development in apoE‐deficient mice in spite of beneficial metabolic effects" Abstract Background and Purpose Fenretinide, a synthetic retinoid derivative first investigated for cancer prevention and treatment, has been shown to ameliorate glucose tolerance, improve plasma lipid profile and reduce body fat mass. These effects, together with its ability to inhibit ceramide synthesis, suggest that fenretinide may have an anti‐atherosclerotic action. Experimental Approach To this aim, nine‐week‐old apoE‐knockout (EKO) female mice were fed for twelve weeks a Western diet, without (control) or with (0.1% w/w) fenretinide. As a reference, wild‐type (WT) mice were treated similarly. Growth and metabolic parameters were monitored throughout the study. Atherosclerosis development was evaluated in the aorta and at the aortic sinus. Blood and lymphoid organs were further characterized with thorough cytological/histological and immunocytofluorimetric analyses. Key Results Fenretinide treatment significantly lowered body weight, glucose levels and plasma levels of total cholesterol, triglycerides, and phospholipids. In the liver, fenretinide remarkably reduced hepatic glycogenosis and steatosis driven by the Western diet. Treated spleens were abnormally enlarged, with severe follicular atrophy and massive extramedullary haematopoiesis. Severe renal hemosiderin deposition was observed in treated EKO mice. Treatment resulted in a threefold increase of total leukocytes (WT and EKO) and raised the activated/resting monocyte ratio in EKO mice. Finally, atherosclerosis development was markedly increased at the aortic arch, thoracic and abdominal aorta of fenretinide‐treated mice. Conclusions and Implications We provide the first evidence that, despite beneficial metabolic effects, fenretinide treatment may enhance the development of atherosclerosis.
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Show moreYear of publication
2021
Authors
Reijo Laaksonen - Creator
Unknown organization
Elena Donetti - Contributor
Eugenio Scanziani - Contributor
Fabrizia Bonacina - Contributor
Francesca Arnaboldi - Contributor
Giulia Chiesa - Contributor
Leonardo Sandrini - Contributor
Marco Busnelli - Contributor
Patrizia Amadio - Contributor
Sabina Soldati - Contributor
Saverio Paltrinieri - Contributor
Silvia Stella Barbieri - Contributor
Stefano Manzini - Contributor
Zenodo - Publisher
Other information
Fields of science
General medicine, internal medicine and other clinical medicine; Pharmacy
Language
English
Open access
Restricted access