FWHM microscopy data

FWHM microscopy data

Description

To measure the optical aberrations caused by different silicone films, we used the full width at half maximum (FWHM) method. 0.2 µm Ø fluorescent FluoSpheres™ Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA were adsorebd to the surface of different silicone membranes by physisorption. These point like objects were imaged with Zeiss LSM 780 confocal microscope with C-Apochromat 40x/1.20 W Korr M27 objective. Z-stacks were taken.1024x1024 pixels, pixel size 103.8 nmZ-step 200nmPixel dwell time 3.9393939393939396e-007 sChannel 1 Argon 488 nmChannel 2 DPSS 561 nm
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Year of publication

2022

Authors

ScienceDB - Publisher

Heidi Peussa - Creator

Other information

Fields of science

Biochemistry, cell and molecular biology; Medical biotechnology

Language

English

Open access

Open

License

Creative Commons Attribution 4.0 International (CC BY 4.0)

Keywords

Medical biotechnology, Biochemistry cell and molecular biology
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