Circulating tumor DNA is a prognostic biomarker in metastatic melanoma patients treated with chemoimmunotherapy and BRAF inhibitor

Circulating tumor DNA is a prognostic biomarker in metastatic melanoma patients treated with chemoimmunotherapy and BRAF inhibitor

Description

Detectable circulating tumor DNA (ctDNA) has been associated with worse prognosis in melanoma patients. We studied plasma ctDNA as a prognostic biomarker in 19 patients with metastatic melanoma and a detectable tumor mutation (13 BRAF, 5 NRAS, and 1 KRAS). Patients had received chemotherapy, interferon-alpha, and vemurafenib in a prospective clinical trial. Mutant allele frequency (MAF %) was determined with droplet digital PCR from pretreatment and sequential plasma samples. Higher pretreatment plasma ctDNA levels (MAF ≥3%) and detectable plasma ctDNA levels (MAF >0%) at the time of radiologically confirmed best objective response were associated with poor prognosis even when accounting for other relevant prognostic factors including performance status, tumor mutation, metastasis stage, and lactate dehydrogenase levels in multivariable analysis. Higher pretreatment plasma ctDNA levels and sustained detectable plasma ctDNA levels during treatment indicated poor prognosis in metastatic melanoma patients.
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Year of publication

2022

Authors

Leena Tiainen - Creator

Meri-Sisko Vuoristo - Creator

Tanja Skyttä - Creator

Unknown organization

Emma Andersson - Creator

Jussi Koivunen - Creator

Kalle E. Mattila - Creator

Kristiina Aittomäki - Creator

Kristiina Tyynelä-Korhonen - Creator

Laura Kohtamäki - Creator

Micaela Hernberg - Creator

Pia Vihinen - Creator

Siru Mäkelä - Creator

Soili Kytölä - Creator

Susan Ramadan - Creator

figshare - Publisher

Other information

Fields of science

Cancers

Language

English

Open access

Open

License

Creative Commons Attribution 4.0 International (CC BY 4.0)

Keywords

Cancers
Circulating tumor DNA is a prognostic biomarker in metastatic melanoma patients treated with chemoimmunotherapy and BRAF inhibitor - Research.fi