Circulating tumor DNA is a prognostic biomarker in metastatic melanoma patients treated with chemoimmunotherapy and BRAF inhibitor
Description
Detectable circulating tumor DNA (ctDNA) has been associated with worse prognosis in melanoma patients. We studied plasma ctDNA as a prognostic biomarker in 19 patients with metastatic melanoma and a detectable tumor mutation (13 BRAF, 5 NRAS, and 1 KRAS). Patients had received chemotherapy, interferon-alpha, and vemurafenib in a prospective clinical trial. Mutant allele frequency (MAF %) was determined with droplet digital PCR from pretreatment and sequential plasma samples. Higher pretreatment plasma ctDNA levels (MAF ≥3%) and detectable plasma ctDNA levels (MAF >0%) at the time of radiologically confirmed best objective response were associated with poor prognosis even when accounting for other relevant prognostic factors including performance status, tumor mutation, metastasis stage, and lactate dehydrogenase levels in multivariable analysis. Higher pretreatment plasma ctDNA levels and sustained detectable plasma ctDNA levels during treatment indicated poor prognosis in metastatic melanoma patients.
Show moreYear of publication
2022
Authors
Leena Tiainen - Creator
Meri-Sisko Vuoristo - Creator
Tanja Skyttä - Creator
Unknown organization
Emma Andersson - Creator
Jussi Koivunen - Creator
Kalle E. Mattila - Creator
Kristiina Aittomäki - Creator
Kristiina Tyynelä-Korhonen - Creator
Laura Kohtamäki - Creator
Micaela Hernberg - Creator
Pia Vihinen - Creator
Siru Mäkelä - Creator
Soili Kytölä - Creator
Susan Ramadan - Creator
figshare - Publisher
Other information
Fields of science
Cancers
Language
English
Open access
Open