Results of surface immunolabeling of KCC2 missense variant p.(Arg231His)

Description

Transient transfection of N2a cells with KCC2 constructs harboring an external pHluorin tag (KCC2-pHext) followed by a live-cell immunostaining protocol used to visualize the surface expressed and internalized pools of KCC2. The data includes fluorescence intensities for human wild-type and variant (p.R231H) KCC2-pHext as well as rat KCC2-pHext and a modified construct whereby the N-terminal has been removed (deltaN rat KCC2-pHext).

Year of publication

2024

Authors

INMED, INSERM, Aix-Marseille University, Marseille, France

Igor Medina - Rights holder, Contributor, Creator

Mira Hamze - Rights holder, Contributor, Creator

University of Oulu

Nano- ja molekyylisysteemien tutkimusyksikkö (NANOMO)

Esa-Ville Immonen - Rights holder, Contributor, Curator

Viivi Järvelä - Rights holder, Curator, Creator, Publisher

Other information

Fields of science

Biochemistry, cell and molecular biology; Neurosciences

Language

English

Open access

Embargo

License

Creative Commons Attribution 4.0 International (CC BY 4.0)

Keywords

KCC2, SLC12A5

Subject headings

neurosciences, cell physiology