README Heliconius erato NMR data The folders contain the raw data output of Nuclear Magnetic Resonance (1H-NMR; using Bruker Avance III HD NMR spectrometer; Bruker BioSpin, Germany) analyses of Heliconius erato butterfly samples. These data have been further analyzed and the results published in the following publications: Mattila ALK, Jiggins CD, Opedal ØH, Montejo-Kovacevich G, Pinheiro de castro ÉC, McMillan WO, Bacquet C, Saastamoinen M. 2021. Evolutionary and ecological processes influencing chemical defense variation in an aposematic and mimetic Heliconius butterfly. PeerJ 9:e11523 https://doi.org/10.7717/peerj.11523 Mattila ALK, Jiggins CD, Saastamoinen M. 2022. Condition dependence in biosynthesized chemical defenses of an aposematic and mimetic Heliconius butterfly. Ecology and Evolution 12 (6), e9041 https://doi.org/10.1002/ece3.9041 In the above publications, the concentrations of the two cyanogenic compounds biosynthesized by Heliconius larvae and adults, linamarin and lotaustralin, were analyzed from the butterfly samples using 1H-NMR data. The peaks of these cyanogen compounds were identified based on comparison of sample 1H spectra with the 1H spectra of authentic reference samples of linamarin and lotaustralin, also included here. The data were processed and analyzed using Bruker TopSpin software (versions 3.2.6; 2014 and 3.5.6; 2016). The key to id´s (NMR_id to Sample_id/Pedigree_data_id), including sample dry mass data and information about sample type can be found in attached file "Heliconius erato NMR data_key to ids.csv". The data folders are named by the NMR_id. The raw data types can be processed and analyzed using Bruker TopSpin software.

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