c-Myc is a highly relevant gene to many cancer types. Currently, there is no treatment method for the c-Myc induced liver cancer, owing to the complicate gene regulation network of c-Myc and difficulty in inactivating c-Myc protein. Promising treatment effect has been shown by inactivating c-Myc gene in mice. CRISPR/Cas9 system has revolutionized the gene editing method. However, the targeted delivery of CRISPR/Cas9 in vivo is still challenging. MSN (mesoporous silica nanoparticles) have high loading capacity for small and macromolecules. Herein, we will load CRISPR/Cas9 into MSN and then encapsulate MSN with a polymer, which is stable in blood but dissolve intracellularly, by microfluidic nanoprecipitation. The polymer will be functionalized with tumor homing ligand glypican-3, and MSN will be conjugated with NLS (nuclear localization signal). We believe that the CRISPR/Cas9 loaded MSN@Polymer will target to the nuclear of cancer cells and treat liver cancer by c-Myc knockout.

2019

2024