Impact of Phytase Treatment and Calcium Addition on Gelation of a Protein-Enriched Rapeseed Fraction
Year of publication
2022
Authors
Silventoinen, Pia; Kortekangas, Anni; Nordlund, Emilia; Sözer, Nesli
Abstract
Rapeseed press cake was upcycled as a protein-enriched ingredient through dry fractionation. The protein-enriched fraction contained higher amounts of phytic acid compared to press cake, and phytase treatment was applied to decrease the phytic acid content from 6.8 to 0.5%. The effect of phytase treatment leading to the release of cations was also mimicked by extrinsic calcium addition. Both phytase treatment and calcium addition significantly improved the heat-induced gel properties but had a minor effect on protein solubility and dispersion stability at pH 8. Water and protein holding capacities of the gels were the highest for the phytase-treated sample (91 and 97%, respectively), followed by the sample with added calcium (86 and 94%, respectively) and control sample (60 and 86%, respectively). Gel firmness followed the same pattern. Scanning electron microscopy images revealed an interconnected structured network in the phytase-treated gel, while in the control gel, a more rigid and open structure was observed. The improved gelation properties resulting from the phytase treatment suggest that the protein and soluble dietary fibre-enriched rapeseed press cake ingredient serve as a promising raw material for gelled food systems. The positive effect of calcium addition on gel properties proposes that part of the improvement observed after phytase treatment may be caused by cations released from phytate.
Show moreOrganizations and authors
VTT Technical Research Centre of Finland Ltd
Kortekangas Anni
Publication type
Publication format
Article
Parent publication type
Journal
Article type
Original article
Audience
ScientificPeer-reviewed
Peer-ReviewedMINEDU's publication type classification code
A1 Journal article (refereed), original researchPublication channel information
Journal
Volume
15
Issue
6
Pages
1422-1435
ISSN
Publication forum
Publication forum level
1
Open access
Open access in the publisher’s service
Yes
Open access of publication channel
Partially open publication channel
License of the publisher’s version
CC BY
Self-archived
No
Other information
Fields of science
Agricultural biotechnology; Environmental biotechnology; Industrial biotechnology
Keywords
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Language
English
International co-publication
No
Co-publication with a company
No
DOI
10.1007/s11947-022-02810-7
The publication is included in the Ministry of Education and Culture’s Publication data collection
Yes