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Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery

Year of publication

2020

Authors

Saari, Heikki; Turunen, Tiia; Lohmus, Andres; Turunen, Mikko; Jalasvuori, Matti; Butcher, Sarah J.; Yla-Herttuala, Seppo; Viitala, Tapani; Cerullo, Vincenzo; Siljander, Pia R. M.; Yliperttula, Marjo

Abstract

Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.
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Organizations and authors

University of Helsinki

Lohmus Andres

Saari Heikki

Yliperttula Marjo

Siljander Pia R. M.

Butcher Sarah J.

Viitala Tapani

Cerullo Vincenzo

University of Jyväskylä

Jalasvuori Matti Orcid -palvelun logo

University of Eastern Finland

Turunen Mikko

Ylä-Herttuala Seppo

Turunen Tiia

Publication type

Publication format

Article

Parent publication type

Journal

Article type

Original article

Audience

Scientific

Peer-reviewed

Peer-Reviewed

MINEDU's publication type classification code

A1 Journal article (refereed), original research

Publication channel information

Volume

9

Issue

1

Pages

1747206

​Publication forum

78431

​Publication forum level

1

Open access

Open access in the publisher’s service

Yes

Open access of publication channel

Fully open publication channel

Self-archived

Yes

Other information

Fields of science

Biochemistry, cell and molecular biology; Biomedicine; Medical biotechnology

Keywords

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Publication country

Sweden

Internationality of the publisher

International

Language

English

International co-publication

No

Co-publication with a company

No

DOI

10.1080/20013078.2020.1747206

The publication is included in the Ministry of Education and Culture’s Publication data collection

Yes