Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching
Year of publication
2020
Authors
Gustavsson, Emil; Isaksson, Linnéa; Persson, Cecilia; Mayzel, Maxim; Brath, Ulrika; Vrhovac, Lidija; Ihalainen, Janne A.; Karlsson, B. Göran; Orekhov, Vladislav; Westenhoff, Sebastian
Abstract
Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although crystal structures of dark and light adapted states have been determined, the molecular mechanisms underlying photoactivation remains elusive. Here we demonstrate that the conserved tongue region of the PHY domain of a 57kDa photosensory module of Deinococcus radiodurans phytochrome, changes from a structurally heterogeneous dark state to an ordered light activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue.
Show moreOrganizations and authors
Publication type
Publication format
Article
Parent publication type
Journal
Article type
Original article
Audience
ScientificPeer-reviewed
Peer-ReviewedMINEDU's publication type classification code
A1 Journal article (refereed), original researchPublication channel information
Journal
Publisher
Volume
118
Issue
2
Pages
415-421
ISSN
Publication forum
Publication forum level
1
Open access
Open access in the publisher’s service
Yes
Open access of publication channel
Delayed open publication channel
Self-archived
Yes
Other information
Fields of science
Biochemistry, cell and molecular biology
Keywords
[object Object],[object Object],[object Object],[object Object]
Publication country
United States
Internationality of the publisher
International
Language
English
International co-publication
Yes
Co-publication with a company
No
DOI
10.1016/j.bpj.2019.11.025
The publication is included in the Ministry of Education and Culture’s Publication data collection
Yes